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ACTIONS
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For colorizing and decolorizing fluorescent images. |
In Actions palette
(Window>Actions), click on the upper, right down arrow and select Load
Actions. (Note that Load Actions is
grayed out and unavailable when an action set is not selected in the Actions
palette). |
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For opening, duplicating, closing the original image, changing to 16-bit mode, and prompting for a history log. F2 key can be used to activate action once the action is loaded. |
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For using indexed color to colorize grayscale images instead of Levels or Curves. |
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ICC PROFILES (TO OBTAIN GAMMA OF
1)
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For obtaining a gamma of 1 from RGB Color files. |
Windows XP: Place file in
C:Windows\System32\Spool\Drivers\Color Mac OS10: Library>ColorSync>Profiles. In Photoshop, in RGB entry in the Color Settings dialog box, select CIE 1931 D65 from drop down list. In the Gray entry, select CIE 1931 Gray. |
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For obtaining a gamma of 1 from Grayscale files. |
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CREDIT for CIE
files: Timo Autiokari:
http://www.aim-dtp.net/aim/ |
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IMAGE FILES |
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Stereology Probes |
Open stereology
probes and use the Transform function (Edit>Transform>Scale) on the
probe layer while holding down the Shift key (to maintain aspect ratio). Fit
probes to desired dimensions. |
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Quantitation sample images |
Use these to practice quantitation and segmentation procedures. |
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Grayscale bar meant for colorizing when making a color bar. |
Include grayscale bar with psuedocolored images so that any adjustment to the image
is shown in the grayscale gradient.
The gradient is 0 - 255 (8-bit) tonal levels, each occupying 10 pixels
of width, with gray level 1 and 3 not included (Photoshop glitch). |
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Periodically spaced dots to test output resolution settings with inkjet printers. |
Print images so that dot spacing
can be visually examined. Banding and
other image flaws can be easily detected.
Print at various output resolutions to test results. See http://www.rags-int-inc.com/PhotoTechStuff/Epson2200/
for more information. |
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Symbols: a selection of various symbols used in science. |
Drag desired symbol from the
appropriate layer. Three resolutions
of each symbol can be chosen. Symbols
include asterisks, open arrowheads, closed arrowheads, etc. |
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SCRIPTS |
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Image size increase or decrease in 10% increments. |
Place Scripts into Windows XP:
C:\Program Files\Adobe\Adobe Photoshop CS3\Presets\Scripts directory. Mac OS10: On a MAC the path would
be: |
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Making a figure from images at identical resolutions |
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Use a filter on all layers |
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CREDIT for Filter
All Visible Layers script: Timo Autiokari:
http://www.aim-dtp.net/aim/ CREDIT for Resampling and Make Figure scripts: Maxime Leveau: http://bipl.umn.edu |
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SUPPLEMENTS TO BOOK |
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For a single PDF file with all supplements described in the “Scientific Imaging with Photoshop” book: All_Supplements.zip
13 megabyte file
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| Downloads | Book: Supplemental Information | Products/Software | Sample Images | Seminars |
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